A strategy for large-scale comparison of evolutionary- and reaction-based classifications of enzyme function.

ABSTRACT: Determining the molecular function of enzymes discovered by genome sequencing represents a primary foundation for understanding many aspects of biology. Historically, classification of enzyme reactions has used the enzyme nomenclature system developed to describe the overall reactions performed by biochemically characterized enzymes, irrespective of their associated sequences. In contrast, functional classification and assignment for the millions of protein sequences of unknown function now available is largely done in two computational steps, first by similarity-based assignment of newly obtained sequences to homologous groups, followed by transferring to them the known functions of similar biochemically characterized homologs. Due to the fundamental differences in their etiologies and pr

SUBMITTER: Holliday GL 

PROVIDER: S-EPMC7246345 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature