Exploring the Enzymatic and Antibacterial Activities of Novel Mycobacteriophage Lysin B Enzymes.

ABSTRACT: Mycobacteriophages possess different sets of lytic enzymes for disruption of the complex cell envelope of the mycobacteria host cells and release of the viral progeny. Lysin B (LysB) enzymes are mycolylarabinogalactan esterases that cleave the ester bond between the arabinogalactan and mycolic acids in the mycolylarabinogalactan-peptidoglycan (mAGP) complex in the cell envelope of mycobacteria. In the present study, four LysB enzymes were produced recombinantly and characterized with respect to their enzymatic and antibacterial activities. Examination of the kinetic parameters for the hydrolysis of para-nitrophenyl ester substrates, shows LysB-His₆ enzymes to be active against a range of substrates (C4-C16), with a catalytic preference towards p-nitrophenyl laurate

SUBMITTER: Abouhmad A 

PROVIDER: S-EPMC7246905 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature