Effects of the scid mutation on X-ray-induced deletions in the brain and spleen of gpt delta mice.

ABSTRACT:

Background

DNA-dependent protein kinase (DNA-PK), consisting of a Ku heterodimer (Ku70/80) and a large catalytic subunit (DNA-PKcs), plays an important role in the repair of DNA double-strand breaks via non-homologous end-joining (NHEJ) in mammalian cells. Severe combined immunodeficient (scid) mice carry a mutation in the gene encoding DNA-PKcs and are sensitive to ionizing radiation. To examine the roles of DNA-PKcs in the generation of deletion mutations in vivo, we crossed scid mice with gpt delta transgenic mice for detecting mutations.

Results

The scid and wild-type (WT) gpt delta transgenic mice were irradiated with a single X-ray dose of 10 Gy, and Spi^- mutant frequencies (MFs) were determined in the brain and spleen 2 da

SUBMITTER: Masumura K 

PROVIDER: S-EPMC7247204 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature