All-in-one sequencing: an improved library preparation method for cost-effective and high-throughput next-generation sequencing.

ABSTRACT:

Background

Next generation sequencing (NGS) has been widely used in biological research, due to its rapid decrease in cost and increasing ability to generate data. However, while the sequence generation step has seen many improvements over time, the library preparation step has not, resulting in low-efficiency library preparation methods, especially for the most time-consuming and labor-intensive steps: size-selection and quantification. Consequently, there can be bottlenecks in projects with large sample cohorts.

Results

We have described the all-in-one sequencing (AIO-seq) method, where instead of performing size-selection and quantification for samples individually, one sample one tube, up to 116 samples are pooled and analyzed in a single tube, 'All-In-One'. The AIO-seq

SUBMITTER: Zhao S 

PROVIDER: S-EPMC7247233 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature