N-terminal protein acetylation by NatB modulates the levels of Nmnats, the NAD⁺ biosynthetic enzymes in Saccharomyces cerevisiae.

ABSTRACT: NAD⁺ is an essential metabolite participating in cellular biochemical processes and signaling. The regulation and interconnection among multiple NAD⁺ biosynthesis pathways are incompletely understood. Yeast (Saccharomyces cerevisiae) cells lacking the N-terminal (Nt) protein acetyltransferase complex NatB exhibit an approximate 50% reduction in NAD⁺ levels and aberrant metabolism of NAD⁺ precursors, changes that are associated with a decrease in nicotinamide mononucleotide adenylyltransferase (Nmnat) protein levels. Here, we show that this decrease in NAD⁺ and Nmnat protein levels is specifically due to the absence of Nt-acetylation of Nmnat (Nma1 and Nma2) proteins and not of other NatB substrates. Nt-acetylation critically reg

SUBMITTER: Croft T 

PROVIDER: S-EPMC7247314 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature