Assay and Isolation of Single Proliferating CD4+ Lymphocytes Using an Automated Microraft Array Platform.

ABSTRACT:

Objective

While T lymphocytes have been employed as a cancer immunotherapy, the development of effective and specific T-cell-based therapeutics remains challenging. A key obstacle is the difficulty in identifying T cells reactive to cancer-associated antigens. The objective of this research was to develop a versatile platform for single cell analysis and isolation that can be applied in immunology research and clinical therapy development.

Methods

An automated microscopy and cell sorting system was developed to track the proliferative behavior of single-cell human primary CD4+ lymphocytes in response to stimulation using allogeneic lymphoblastoid feeder cells.

Results

The system identified single human T lymphocytes with a sensitivity of 98% and specificity of 99% and

SUBMITTER: LaBelle CA 

PROVIDER: S-EPMC7247929 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature