Wnt10b-overexpressing umbilical cord mesenchymal stem cells promote critical size rat calvarial defect healing by enhanced osteogenesis and VEGF-mediated angiogenesis.

ABSTRACT:

Background/objectives

Accelerating the process of bone regeneration is of great interest for surgeons and basic scientists alike. Recently, umbilical cord mesenchymal stem cells (UCMSCs) are considered clinically applicable for tissue regeneration due to their noninvasive harvesting and better viability. Nonetheless, the bone regenerative ability of human UCMSCs (HUCMSCs) is largely unknown. This study aimed to investigate whether Wnt10b-overexpressing HUCMSCs have enhanced bone regeneration ability in a rat model.

Method

A rat calvarial defect was performed on 8-week old male Sprague Dawley rats. Commercially purchased HUCMSCs^Emp in hydrogel, HUCMSCs^Wnt10b in hydrogel and HUCMSCs^Wnt10b with IWR-1 were placed in the calvarial bone defect righ

SUBMITTER: Liu Y 

PROVIDER: S-EPMC7248289 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature