Immobilized Lipase in the Synthesis of High Purity Medium Chain Diacylglycerols Using a Bubble Column Reactor: Characterization and Application.

ABSTRACT: Novozym^® 435, an immobilized lipase from Candida antarctica B. (CALB), was used as a biocatalyst for the synthesis of high purity medium chain diacylglycerol (MCD) in a bubble column reactor. In this work, the properties of the MCD produced were characterized followed by determining its practical application as an emulsifier in water-in-oil (W/O) emulsion. Two types of MCDs, namely, dicaprylin (C₈-DAG) and dicaprin (C₁₀-DAG), were prepared through enzymatic esterification using the following conditions: 5% Novozym^® 435, 2.5% deionized water, 60°C for 30 min followed by purification. A single-step molecular distillation (MD) (100-140°C, 0.1 Pa, 300 rpm) was performed and comparison was made to that of a double-step purification with MD foll

SUBMITTER: Chen J 

PROVIDER: S-EPMC7248569 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature