Microsatellite marker development in the crop wild relative Linum bienne using genome skimming.

ABSTRACT:

Premise

Nuclear microsatellite markers were developed for Linum bienne, the sister species of the crop L. usitatissimum, to provide molecular genetic tools for the investigation of L. bienne genetic diversity and structure.

Methods and results

Fifty microsatellite loci were identified in L. bienne by means of genome skimming, and 44 loci successfully amplified. Of these, 16 loci evenly spread across the L. usitatissimum reference nuclear genome were used for genotyping six L. bienne populations. Excluding one monomorphic locus, the number of alleles per locus ranged from two to 12. Four out of six populations harbored private alleles. The levels of expected and observed heterozygosity were 0.076 to 0.667 and 0.000 to 1.000, respectiv

SUBMITTER: Landoni B 

PROVIDER: S-EPMC7249271 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature