Development and characterization of EST-SSR markers for Camellia reticulata.

ABSTRACT:

Premise

Camellia reticulata, which is native to southwestern China, is an economically important plant belonging to the family Theaceae. We developed expressed sequence tag-simple sequence repeat (EST-SSR) markers for C. reticulata, which can be used to investigate its genetic diversity, population structure, and evolutionary history.

Methods and results

We detected 4780 SSRs in C. reticulata from Camellia RNA-Seq data deposited in the National Center for Biotechnology Information's expressed sequence tags database (dbEST). Primer pairs for 70 SSR loci were designed and used for PCR amplification using 90 individuals from four populations of C. reticulata. Of these loci, 50 microsatellite markers were successfully identified, including 11 p

SUBMITTER: Tong Y 

PROVIDER: S-EPMC7249272 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature