Purification and production of Plasmodium falciparum zygotes from in vitro culture using magnetic column and Percoll density gradient.

ABSTRACT:

Background

Plasmodium falciparum zygotes develop in the mosquito midgut after an infectious blood meal containing mature male and female gametocytes. Studies of mosquito-produced P. falciparum zygotes to elucidate their biology and development have been hampered by high levels of contaminating mosquito proteins and macromolecules present in zygote preparations. Thus, no zygote-specific surface markers have been identified to date. Here, a methodology is developed to obtain large quantities of highly purified zygotes using in vitro culture, including purification methods that include magnetic column cell separation (MACS) followed by Percoll density gradient centrifugation. This straightforward and effective approach provides ample material for studies to enhance understanding of zy

SUBMITTER: Zhou Y 

PROVIDER: S-EPMC7249376 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature