ARTDeco: automatic readthrough transcription detection.

ABSTRACT:

Background

Mounting evidence suggests several diseases and biological processes target transcription termination to misregulate gene expression. Disruption of transcription termination leads to readthrough transcription past the 3' end of genes, which can result in novel transcripts, changes in epigenetic states and altered 3D genome structure.

Results

We developed Automatic Readthrough Transcription Detection (ARTDeco), a tool to detect and analyze multiple features of readthrough transcription from RNA-seq and other next-generation sequencing (NGS) assays that profile transcriptional activity. ARTDeco robustly quantifies the global severity of readthrough phenotypes, and reliably identifies individual genes that fail to terminate (readthrough genes), are aberrantly transcr

SUBMITTER: Roth SJ 

PROVIDER: S-EPMC7249449 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature