Quantitative Imprint Mass Spectrometry Imaging of Endogenous Ceramides in Rat Brain Tissue with Kinetic Calibration.

ABSTRACT: Quantitative mass spectrometry imaging (MSI) is an effective technique for determining the spatial distribution of molecules in a variety of sample types; however, the quality of the ion signals is related to the chemical and morphological properties of the tissue and the targeted analyte(s). Issues may arise with the incorporation of standards into the tissue at repeatable, well-defined concentrations, as well as with the extraction and incorporation of endogenous analytes versus standards from tissue into the matrix. To address these concerns, we combine imprint MSI (iMSI) with kinetic calibration and use it to quantify lipids in rat brain tissue samples. Briefly, tissues were imprinted on slides coated with a dopamine-modified TiO₂ monolith pretreated with analyte standards,

SUBMITTER: Wu Q 

PROVIDER: S-EPMC7250146 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature