Prevalence and distribution of antimicrobial resistance determinants of Escherichia coli isolates obtained from meat in South Africa.

ABSTRACT:

Objective

This study aimed to characterise antibiotics resistance of Escherichia coli isolates from the formal meat sector (FMS) and informal meat sectors (INMS).

Method

A total of 162 and 102 E. coli isolates from the FMS, and INMS respectively were isolated by standard culture-based, and biochemical reactions. The isolates were further confirmed by polymerase chain reaction (PCR). The disc diffusion method was used to screen for antimicrobial susceptibility against 19 different antibiotics. The presence of class 1-2 integrons in each E. coli isolates was assessed using 3'-CS and 5'-CS regions specific primers.

Result

Among the 19 antimicrobials, resistance to tetracyclines, aminoglycosides, cephalosporins, and nitrofurans were found to be more frequent than carbapen

SUBMITTER: Jaja IF 

PROVIDER: S-EPMC7250413 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature