Accumulation of ambient phosphate into the periplasm of marine bacteria is proton motive force dependent.

ABSTRACT: Bacteria acquire phosphate (P_i) by maintaining a periplasmic concentration below environmental levels. We recently described an extracellular P_i buffer which appears to counteract the gradient required for P_i diffusion. Here, we demonstrate that various treatments to outer membrane (OM) constituents do not affect the buffered P_i because bacteria accumulate P_i in the periplasm, from which it can be removed hypo-osmotically. The periplasmic P_i can be gradually imported into the cytoplasm by ATP-powered transport, however, the proton motive force (PMF) is not required to keep P_i in the periplasm. In contrast, the accumulation of P_i into the periplasm across the OM is PMF-dependent and can be enhanced by li

SUBMITTER: Kamennaya NA 

PROVIDER: S-EPMC7250820 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature