Role of Small Intestine and Gut Microbiome in Plant-Based Oral Tolerance for Hemophilia.

ABSTRACT: Fusion proteins, which consist of factor VIII or factor IX and the transmucosal carrier cholera toxin subunit B, expressed in chloroplasts and bioencapsulated within plant cells, initiate tolerogenic immune responses in the intestine when administered orally. This approach induces regulatory T cells (Treg), which suppress inhibitory antibody formation directed at hemophilia proteins induced by intravenous replacement therapy in hemophilia A and B mice. Further analyses of Treg CD4⁺ lymphocyte sub-populations in hemophilia B mice reveal a marked increase in the frequency of CD4⁺CD25^-FoxP3^-LAP⁺ T cells (but not of CD4⁺CD25⁺FoxP3⁺ T cells) in the lamina propria of the small but not large intestine. The ado

SUBMITTER: Kumar SRP 

PROVIDER: S-EPMC7251037 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature