How Do You Identify m⁶ A Methylation in Transcriptomes at High Resolution? A Comparison of Recent Datasets.

ABSTRACT: A flurry of methods has been developed in recent years to identify N6-methyladenosine (m⁶A) sites across transcriptomes at high resolution. This raises the need to understand both the common features and those that are unique to each method. Here, we complement the analyses presented in the original papers by reviewing their various technical aspects and comparing the overlap between m⁶A-methylated messenger RNAs (mRNAs) identified by each. Specifically, we examine eight different methods that identify m⁶A sites in human cells with high resolution: two antibody-based crosslinking and immunoprecipitation (CLIP) approaches, two using endoribonuclease MazF, one based on deamination, two using Nanopore direct RNA sequencing, and finally, one based on computatio

SUBMITTER: Capitanchik C 

PROVIDER: S-EPMC7251061 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature