G3 and G9 Rotavirus genotypes in waste water circulation from two major metropolitan cities of Pakistan.

ABSTRACT: Rotavirus A (RVA) is a diarrheal pathogen affecting children under age five, particularly in developing and underdeveloped regions of the world due to malnutrition, poor healthcare and hygienic conditions. Water and food contamination are found to be major sources of diarrheal outbreaks. Pakistan is one of the countries with high RVA related diarrhea burden but with insufficient surveillance system. The aim of this study was to gauge the RVA contamination of major open sewerage collecting streams and household water supplies in two major metropolitan cities of Pakistan. Three concentration methods were compared using RNA purity and concentration as parameters, and detection efficiency of the selected method was estimated. Water samples were collected from 21 sites in Islamabad and Rawalpin

SUBMITTER: Naqvi SS 

PROVIDER: S-EPMC7251132 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature