LPS-Treated Podocytes Polarize Naive CD4⁺ T Cells into Th17 and Treg Cells.

ABSTRACT:

Aim

Our study is aimed at investigating whether Lipopolysaccharide- (LPS-) treated podocytes could polarize naive CD4⁺ T cells into different subsets in vitro.

Materials and methods

Podocytes and mouse bone marrow-derived dendritic cells (BMDCs) were first cultured with 25 μg/ml LPS for 6 hours, respectively. Then, naive CD4⁺ T cells were cocultured with the LPS-treated podocytes or BMDCs at a ratio of 1 : 1 or 1 : 1 : 1. After 48 hours, we collected the suspended cells and supernatant from all groups to measure T helper (Th)17 cells, regulatory T (Treg) cells, and cytokine concentration.

Results

We observed the expression of CD80 and major histocompatibility complex class II molecule (MHC II) in podocytes but did not found the upregulatio

SUBMITTER: Yuan DH 

PROVIDER: S-EPMC7251438 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature