Enhanced production of recombinant proteins in Corynebacterium glutamicum by constructing a bicistronic gene expression system.

ABSTRACT:

Background

Corynebacterium glutamicum is a traditional food-grade industrial microorganism, in which an efficient endotoxin-free recombinant protein expression factory is under developing in recent years. However, the intrinsic disadvantage of low recombinant protein expression level is still difficult to be solved. Here, according to the bacteria-specific polycistronic feature that multiple proteins can be translated in one mRNA, efforts have been made to insert a leading peptide gene upstream of target genes as an expression enhancer, and it is found that this can remarkably improve the expression level of proteins under the control of inducible tac promoter in C. glutamicum.

Results

In this research, the Escherichia coli (E. coli) tac promoter combined with 24 different f

SUBMITTER: Sun M 

PROVIDER: S-EPMC7251831 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature