Construction of reporter gene assays using CWP and PDR mutant yeasts for enhanced detection of various sex steroids.

ABSTRACT:

Background

Sex steroid hormone receptors are classified into three classes of receptors: estrogen receptors (ER) α and β, androgen receptor (AR), and progesterone receptor (PR). They belong to the nuclear receptor superfamily and activate their downstream genes in a ligand-dependent manner. Since sex steroid hormones are involved in a wide variety of physiological processes and cancer development, synthetic chemical substances that exhibit sex steroid hormone activities have been applied as pharmaceuticals and consumed in large amounts worldwide. They are potentially hazardous contaminants as endocrine disruptors in the environment because they may induce inappropriate gene expression mediated by sex steroid hormone receptors in vivo.

Results

To develop simple reporter gene

SUBMITTER: Ito-Harashima S 

PROVIDER: S-EPMC7251871 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature