Wax ester production in nitrogen-rich conditions by metabolically engineered Acinetobacter baylyi ADP1.

ABSTRACT: Metabolic engineering can be used as a powerful tool to redirect cell resources towards product synthesis, also in conditions that are not optimal for the production. An example of synthesis strongly dependent on external conditions is the production of storage lipids, which typically requires a high carbon/nitrogen ratio. This requirement also limits the use of abundant nitrogen-rich materials, such as industrial protein by-products, as substrates for lipid production. Acinetobacter baylyi ADP1 is known for its ability to produce industrially interesting storage lipids, namely wax esters (WEs). Here, we engineered A. baylyi ADP1 by deleting the gene aceA encoding for isocitrate lyase and overexpressing fatty acyl-CoA reductase Acr1 in the wax ester production pathway to allow redirection

SUBMITTER: Luo J 

PROVIDER: S-EPMC7251950 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature