Identification of differentially expressed genes between the colon and ileum of patients with inflammatory bowel disease by gene co-expression analysis.

ABSTRACT:

Objective

We aimed to identify differentially expressed genes (DEG) in patients with inflammatory bowel disease (IBD).

Methods

RNA-seq data were obtained from the Array Express database. DEG were identified using the edgeR package. A co-expression network was constructed and key modules with the highest correlation with IBD inflammatory sites were identified for analysis. The Cytoscape MCODE plugin was used to identify key sub-modules of the protein-protein interaction (PPI) network. The genes in the sub-modules were considered hub genes, and functional enrichment analysis was performed. Furthermore, we constructed a drug-gene interaction network. Finally, we visualized the hub gene expression pattern between the colon and ileum of IBD using the ggpubr package and analyzed i

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC7251957 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature