DNA methylation dynamics underlie metamorphic gene regulation programs in Xenopus tadpole brain.

ABSTRACT: Methylation of cytosine residues in DNA influences chromatin structure and gene transcription, and its regulation is crucial for brain development. There is mounting evidence that DNA methylation can be modulated by hormone signaling. We analyzed genome-wide changes in DNA methylation and their relationship to gene regulation in the brain of Xenopus tadpoles during metamorphosis, a thyroid hormone-dependent developmental process. We studied the region of the tadpole brain containing neurosecretory neurons that control pituitary hormone secretion, a region that is highly responsive to thyroid hormone action. Using Methylated DNA Capture sequencing (MethylCap-seq) we discovered a diverse landscape of DNA methylation across the tadpole neural cell genome, and pairwise stage comparisons identi

SUBMITTER: Kyono Y 

PROVIDER: S-EPMC7251973 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature