Iodine-124 Based Dual Positron Emission Tomography and Fluorescent Labeling Reagents for In Vivo Cell Tracking.

ABSTRACT: Understanding the in vivo behavior of experimental therapeutic cells is fundamental to their successful development and clinical translation. Iodine-124 has the longest half-life (4.2 days) among the clinically used positron emitters. Consequently, this isotope offers the longest possible tracking time for directly labeled cells using positron emission tomography (PET). Herein, we have radiosynthesized and evaluated two iodine-124/fluorescein-based dual PET and fluorescent labeling reagents, namely ¹²⁴I-FIT-Mal and ¹²⁴I-FIT-(PhS)₂Mal for cell surface thiol bioconjugation. ¹²⁴I-FIT-(PhS)₂Mal labeled cells significantly more effectively than ¹²⁴I-FIT-Mal. It conjugated to various cell lines in 22%-62% labeling efficienc

SUBMITTER: Pham TT 

PROVIDER: S-EPMC7252900 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature