Identification of Lineage-Specific Transcription Factors That Prevent Activation of Hepatic Stellate Cells and Promote Fibrosis Resolution.

ABSTRACT:

Background & aims

Development of liver fibrosis is associated with activation of quiescent hepatic stellate cells (HSCs) into collagen type I-producing myofibroblasts (activated HSCs). Cessation of liver injury often results in fibrosis resolution and inactivation of activated HSCs/myofibroblasts into a quiescent-like state (inactivated HSCs). We aimed to identify molecular features of phenotypes of HSCs from mice and humans.

Methods

We performed studies with Lrat^Cre, Ets1-floxed, Nf1-floxed, Pparγ-floxed, Gata6-floxed, Rag2^-/-γc^-/-, and C57/Bl6 (control) mice. Some mice were given carbon tetrachloride (CCl₄) to induce liver fibrosis, with or without a peroxisome proliferator-activated receptor-γ (PPARγ) agonist. Livers from mice w

SUBMITTER: Liu X 

PROVIDER: S-EPMC7252905 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature