Metabolomics Benefits from Orbitrap GC-MS-Comparison of Low- and High-Resolution GC-MS.

ABSTRACT: The development of improved mass spectrometers and supporting computational tools is expected to enable the rapid annotation of whole metabolomes. Essential for the progress is the identification of strengths and weaknesses of novel instrumentation in direct comparison to previous instruments. Orbitrap liquid chromatography (LC)-mass spectrometry (MS) technology is now widely in use, while Orbitrap gas chromatography (GC)-MS introduced in 2015 has remained fairly unexplored in its potential for metabolomics research. This study aims to evaluate the additional knowledge gained in a metabolomics experiment when using the high-resolution Orbitrap GC-MS in comparison to a commonly used unit-mass resolution single-quadrupole GC-MS. Samples from an osmotic stress treatment of a non-model organis

SUBMITTER: Stettin D 

PROVIDER: S-EPMC7254393 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature