Comparing Cryo-EM Reconstructions and Validating Atomic Model Fit Using Difference Maps.

ABSTRACT: Cryogenic electron microscopy (cryo-EM) is a powerful technique for determining structures of multiple conformational or compositional states of macromolecular assemblies involved in cellular processes. Recent technological developments have led to a leap in the resolution of many cryo-EM data sets, making atomic model building more common for data interpretation. We present a method for calculating differences between two cryo-EM maps or a map and a fitted atomic model. The proposed approach works by scaling the maps using amplitude matching in resolution shells. To account for variability in local resolution of cryo-EM data, we include a procedure for local amplitude scaling that enables appropriate scaling of local map contrast. The approach is implemented as a user-friendly tool in the

SUBMITTER: Joseph AP 

PROVIDER: S-EPMC7254831 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature