Could Egg White Lysozyme be Solved by Single Particle Cryo-EM?

ABSTRACT: The combination of high-end cryogenic transmission electron microscopes (cryo-EM), direct electron detectors, and advanced image algorithms allows researchers to obtain the 3D structures of much smaller macromolecules than years ago. However, there are still major challenges for the single-particle cryo-EM method to achieve routine structure determinations for macromolecules much smaller than 100 kDa, which are the majority of all plant and animal proteins. These challenges include sample characteristics such as sample heterogeneity, beam damage, ice layer thickness, stability, and quality, as well as hardware limitations such as detector performance, beam, and phase plate quality. Here, single particle data sets were simulated for samples that were ideal in terms of homogeneity, distribut

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC7254834 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature