Sample preparation strategies for high-throughput mass spectrometry imaging of primary tumor organoids.

ABSTRACT: Patient-derived 3D organoids show great promise for understanding patient heterogeneity and chemotherapy response in human-derived tissue. The combination of organoid culture techniques with mass spectrometry imaging provides a label-free methodology for characterizing drug penetration, patient-specific response, and drug biotransformation. However, current methods used to grow tumor organoids employ extracellular matrices that can produce small molecule background signal during mass spectrometry imaging analysis. Here, we develop a method to isolate 3D human tumor organoids out of a Matrigel extracellular matrix into gelatin mass spectrometry compatible microarrays for high-throughput mass spectrometry imaging analysis. The alignment of multiple organoids in the same z-axis is essential f

SUBMITTER: Johnson J 

PROVIDER: S-EPMC7254934 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature