DR-region of Na⁺/K⁺-ATPase is a target to ameliorate hepatic insulin resistance in obese diabetic mice.

ABSTRACT: Reduced hepatic Na⁺/K⁺-ATPase (NKA) activity and NKAα1 expression are engaged in the pathologies of metabolism diseases. The present study was designed to investigate the potential roles of NKAα1 in hepatic gluconeogenesis and glycogenesis in both hepatocytes and obese diabetic mice. Methods: Insulin resistance was mimicked by glucosamine (GlcN) in either human hepatocellular carcinoma (HepG2) cells or primary mouse primary hepatocytes. Obese diabetic mice were induced by high-fat diet (HFD) feeding for 12 weeks. Results: We found that both NKA activity and NKAα1 protein level were downregulated in GlcN-treated hepatocytes and in the livers of obese diabetic mice. Pharmacological inhibition of NKA with ouabain worsened, while activation of NKAα1 with an

SUBMITTER: Sun HJ 

PROVIDER: S-EPMC7255017 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature