Kindlin-2 deficiency induces fatal intestinal obstruction in mice.

ABSTRACT: Rationale: Smooth muscle-motility disorders are mainly characterized by impaired contractility and functional intestinal obstruction. Some of these cases are caused by genetic mutations of smooth muscle genes ACTA2, ACTG2, MYH11, MYLK and LMOD1. Still the etiology is complex and multifactorial and the underlying pathology is poorly understood. Integrin interaction protein Kindlin-2 is widely expressed in striated and smooth muscle cells (SMC). However, the function of Kindlin-2 in the smooth muscle remains elusive. Methods: We generated two mouse models using different cre promoter transgenic mice, Kindlin-2^fl/fl SM22α-cre+ (cKO mice) and Kindlin-2^fl/fl; MYH-cre+ (iKO mice). Embryos and adult tissues were prepared for hematoxylin and eosin (H&E) staining

SUBMITTER: He X 

PROVIDER: S-EPMC7255029 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature