Deep Proteomics Using Two Dimensional Data Independent Acquisition Mass Spectrometry.

ABSTRACT: Methodologies that facilitate high-throughput proteomic analysis are a key step toward moving proteome investigations into clinical translation. Data independent acquisition (DIA) has potential as a high-throughput analytical method due to the reduced time needed for sample analysis, as well as its highly quantitative accuracy. However, a limiting feature of DIA methods is the sensitivity of detection of low abundant proteins and depth of coverage, which other mass spectrometry approaches address by two-dimensional fractionation (2D) to reduce sample complexity during data acquisition. In this study, we developed a 2D-DIA method intended for rapid- and deeper-proteome analysis compared to conventional 1D-DIA analysis. First, we characterized 96 individual fractions obtained from the protei

SUBMITTER: Cho KC 

PROVIDER: S-EPMC7255061 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature