Superresolution imaging of chromatin fibers to visualize epigenetic information on replicative DNA.

ABSTRACT: During DNA replication, the genetic information of a cell is copied. Subsequently, identical genetic information is segregated reliably to the two daughter cells through cell division. Meanwhile, DNA replication is intrinsically linked to the process of chromatin duplication, which is required for regulating gene expression and establishing cell identities. Understanding how chromatin is established, maintained or changed during DNA replication represents a fundamental question in biology. Recently, we developed a method to directly visualize chromatin components at individual replication forks undergoing DNA replication. This method builds upon the existing chromatin fiber technique and combines it with cell type-specific chromatin labeling and superresolution microscopy. In this method,

SUBMITTER: Wooten M 

PROVIDER: S-EPMC7255620 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature