Circulating microRNA as biomarkers of canine mammary carcinoma in dogs.

ABSTRACT:

Background

Differentiating benign from canine malignant mammary tumors requires invasive surgical biopsy. Circulating microRNAs (miRNA) may represent promising minimally invasive cancer biomarkers in people and animals.

Objectives

To evaluate the serum mRNA profile between dogs with and without mammary carcinoma, and to determine if any of these markers have prognostic significance.

Animals

Ten healthy client-owned female dogs (5 intact, 5 spayed) and 10 dogs with histologically confirmed mammary carcinoma were included; 9 were client-owned, whereas 1 was a research colony dog.

Methods

Retrospective study. Serum miRNA was evaluated by RNA deep-sequencing (RNAseq) and digital droplet PCR (dPCR).Expression of candidate biomarkers miR-18a, miR-19b, miR-29b, miR-34

SUBMITTER: Fish EJ 

PROVIDER: S-EPMC7255679 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature