Nephron loss detected by MRI following neonatal acute kidney injury in rabbits.

ABSTRACT:

Background

Acute kidney injury affects nearly 30% of preterm neonates in the intensive care unit. We aimed to determine whether nephrotoxin-induced AKI disrupted renal development assessed by imaging (CFE-MRI).

Methods

Neonatal New Zealand rabbits received indomethacin and gentamicin (AKI) or saline (control) for four days followed by cationic ferritin (CF) at six weeks. Ex vivo images were acquired using a gradient echo pulse sequence on 7 T MRI. Glomerular number (N_glom) and apparent glomerular volume (aV_glom) were determined. CF toxicity was assessed at two and 28 days in healthy rabbits.

Results

N_glom was lower in the AKI group as compared to controls (74,034 vs 198,722, p < 0.01). aV_glom was not different (AKI: 7.3 × 10

SUBMITTER: Charlton JR 

PROVIDER: S-EPMC7255918 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature