Pituispheres Contain Genetic Variants Characteristic to Pituitary Adenoma Tumor Tissue.

ABSTRACT: The most common type of pituitary neoplasms is benign pituitary adenoma (PA). Clinically significant PAs affect around 0.1% of the population. Currently, there is no established human PA cell culture available and when PA tumor cells are cultured they form two distinct types depending on culturing conditions either free-floating aggregates also known as pituispheres or cells adhering to the surface of cell plates and displaying mesenchymal stem-like properties. The aim of this study was to trace the origin of sphere-forming and adherent pituitary cell cultures and characterize the potential use of these surgery derived cell lines as PA model. We carried out a paired-end exome sequencing of patients' tumor and germline DNA using Illumina NextSeq followed by characterization of corresponding

SUBMITTER: Peculis R 

PROVIDER: S-EPMC7256168 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature