Real-time RT-PCR assay to detect Granada virus and the related Massilia and Arrabida phleboviruses.

ABSTRACT:

Background

Granada virus belongs to the genus Phlebovirus within the Naples serocomplex and was detected for the first time in sand flies from Spain in 2003. Seroprevalence studies have revealed that Granada virus may infect humans with most cases being asymptomatic. Moreover, recent studies in vector samples revealed that the related Massilia and Arrabida phleboviruses could be also circulating in Spain. The objective of this study was to develop and assess a new sensitive real-time RT-PCR assay for Granada virus diagnosis able to detect the related phleboviruses Massilia and Arrabida.

Methods

Two specific primers and one unique probe to detect Granada, Massilia and Arrabida viruses, without differentiating between them, were designed targeting the conserved L-segment of th

SUBMITTER: Davo L 

PROVIDER: S-EPMC7257231 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature