Novel role of PAF1 in attenuating radiosensitivity in cervical cancer by inhibiting IER5 transcription.

ABSTRACT:

Background

Radiosensitivity is limited in cervical cancer (CC) patients due to acquired radiation resistance. In our previous studies, we found that immediate-early response 5 (IER5) is upregulated in CC cells upon radiation exposure and decreases cell survival by promoting apoptosis. The details on the transcriptional regulation of radiation-induced IER5 expression are unknown. Studies in recent years have suggested that Pol II-associated factor 1 (PAF1) is a pivotal transcription factor for certain genes "induced" during tumor progression. In this study, we investigated the role of PAF1 in regulating IER5 expression during CC radiotherapy.

Methods

PAF1 expression in CC cells was measured by western blotting, immunohistochemistry, and qRT-PCR, and the localization of PAF1 a

SUBMITTER: Zheng JJ 

PROVIDER: S-EPMC7257241 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature