A novel NGS-based microsatellite instability (MSI) status classifier with 9 loci for colorectal cancer patients.

ABSTRACT:

Background

With the recent emergence of immune checkpoint inhibitors, microsatellite instability (MSI) status has become an important biomarker for immune checkpoint blockade therapy. There are growing technical demands for the integration of different genomic alterations profiling including MSI analysis in a single assay for full use of the limited tissues.

Methods

Tumor and paired control samples from 64 patients with primary colorectal cancer were enrolled in this study, including 14 MSI-high (MSI-H) cases and 50 microsatellite stable (MSS) cases determined by MSI-PCR. All the samples were sequenced by a customized NGS panel covering 2.2 MB. A training dataset of 28 samples was used for selection of microsatellite loci and a novel NGS-based MSI status classifier, USCI-msi

SUBMITTER: Zheng K 

PROVIDER: S-EPMC7257555 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature