Novel morphological multi-scale evaluation system for quality assessment of decellularized liver scaffolds.

ABSTRACT: Decellularized scaffolds can serve as an excellent three-dimensional environment for cell repopulation. They maintain tissue-specific microarchitecture of extracellular matrix proteins with important spatial cues for cell adhesion, migration, growth, and differentiation. However, criteria for quality assessment of the three-dimensional structure of decellularized scaffolds are rather fragmented, usually study-specific, and mostly semi-quantitative. Thus, we aimed to develop a robust structural assessment system for decellularized porcine liver scaffolds. Five scaffolds of different quality were used to establish the new evaluation system. We combined conventional semi-quantitative scoring criteria with a quantitative scaffold evaluation based on automated image analysis. For the quantitati

SUBMITTER: Moulisova V 

PROVIDER: S-EPMC7257850 | biostudies-literature | 2020 Jan-Dec

REPOSITORIES: biostudies-literature