Phenotypic Diversity Caused by Differential Expression of SFTPC-Cre-Transgenic Alleles.

ABSTRACT: Type II alveolar epithelial cells (AEC2s) play an essential role in the function and maintenance of the pulmonary epithelium. Several transgenic mice have been developed to study the function of these cells in vivo by using the human SFTPC promoter to drive expression of Cre recombinase. The precise activity of each of these transgenic alleles has not been studied, and previous reports suggest that their activity can depend on breeding strategies. We bred mice with a conditional allele of the essential telomere capping protein TRF2 with two different SFTPC-Cre-transgenic strains and observed opposite phenotypes (100% lethality vs. 100% viability). We characterized the Cre recombinase activity in these two transgenic lines and found that the contrasting phenotypes were

SUBMITTER: Jiang M 

PROVIDER: S-EPMC7258818 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature