Nano-enhanced Optical Gene Delivery to Retinal Degenerated Mice.

ABSTRACT: BACKGROUND:The efficient and targeted delivery of genes and other impermeable therapeutic molecules into retinal cells is of immense importance for the therapy of various visual disorders. Traditional methods for gene delivery require viral transfection, or chemical methods that suffer from one or many drawbacks, such as low efficiency, lack of spatially targeted delivery, and can generally have deleterious effects, such as unexpected inflammatory responses and immunological reactions. METHODS:We aim to develop a continuous wave near-infrared laser-based Nano-enhanced Optical Delivery (NOD) method for spatially controlled delivery of ambient-light-activatable Muti-Characteristic opsin-encoding genes into retina in-vivo and ex-vivo. In this method, the optical field enhancement by gold nano

SUBMITTER: Batabyal S 

PROVIDER: S-EPMC7258984 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature