A novel stable isotope tracer method to simultaneously quantify skeletal muscle protein synthesis and breakdown.

ABSTRACT:

Background/aims

Methodological challenges have been associated with the dynamic measurement of muscle protein breakdown (MPB), as have the measurement of both muscle protein synthesis (MPS) and MPB within the same experiment. Our aim was to use the transmethylation properties of methionine as proof-of-concept to measure rates of MPB via its methylation of histidine within skeletal muscle myofibrillar proteins, whilst simultaneously utilising methionine incorporation into bound protein to measure MPS.

Results

During the synthesis measurement period, incorporation of methyl[D₃]-¹³C-methionine into cellular protein in C2C12 myotubes was observed (representative of MPS), alongside an increase in the appearance of methyl[D₃]-methylhistidine into t

SUBMITTER: Crossland H 

PROVIDER: S-EPMC7259457 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature