Integrated human pseudoislet system and microfluidic platform demonstrate differences in GPCR signaling in islet cells.

ABSTRACT: Pancreatic islets secrete insulin from β cells and glucagon from α cells, and dysregulated secretion of these hormones is a central component of diabetes. Thus, an improved understanding of the pathways governing coordinated β and α cell hormone secretion will provide insight into islet dysfunction in diabetes. However, the 3D multicellular islet architecture, essential for coordinated islet function, presents experimental challenges for mechanistic studies of intracellular signaling pathways in primary islet cells. Here, we developed an integrated approach to study the function of primary human islet cells using genetically modified pseudoislets that resemble native islets across multiple parameters. Further, we developed a microperifusion system that allowed synchronous acquisition of GC

SUBMITTER: Walker JT 

PROVIDER: S-EPMC7259531 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature