PFKP is transcriptionally repressed by BRCA1/ZBRK1 and predicts prognosis in breast cancer.

ABSTRACT: OBJECTIVES:The present study aims to elucidate the underlying mechanism how PFKP is regulated by BRCA1 and the clinical significance of PFKP in breast cancer. METHODS:MEF-BRCA1△/△ and the wild type counterpart MEF-BRCA1+/+ cell lines were used to test the sensitivity of glucose depletion in culture medium. Glucose Assay Kit was used to quantify glucose levels in cultural supernatant and cell lysate. Real time PCR was used to measure the mRNA expression levels of genes. Western blot was used to detect protein levels. Chromatin immunoprecipitation was used to verify the bindings between transcription factors and DNA elements. Luciferase reporter assay was performed to determine the transcriptional activity. Histochemistry assay was performed on tissue microarray. RESULTS:We found that MEF-BR

SUBMITTER: Yeerken D 

PROVIDER: S-EPMC7259711 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature