Humanization of yeast genes with multiple human orthologs reveals functional divergence between paralogs.

ABSTRACT: Despite over a billion years of evolutionary divergence, several thousand human genes possess clearly identifiable orthologs in yeast, and many have undergone lineage-specific duplications in one or both lineages. These duplicated genes may have been free to diverge in function since their expansion, and it is unclear how or at what rate ancestral functions are retained or partitioned among co-orthologs between species and within gene families. Thus, in order to investigate how ancestral functions are retained or lost post-duplication, we systematically replaced hundreds of essential yeast genes with their human orthologs from gene families that have undergone lineage-specific duplications, including those with single duplications (1 yeast gene to 2 human genes, 1:2) or higher-order expans

SUBMITTER: Laurent JM 

PROVIDER: S-EPMC7259792 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature