Discovery of genes required for body axis and limb formation by global identification of retinoic acid-regulated epigenetic marks.

ABSTRACT: Identification of target genes that mediate required functions downstream of transcription factors is hampered by the large number of genes whose expression changes when the factor is removed from a specific tissue and the numerous binding sites for the factor in the genome. Retinoic acid (RA) regulates transcription via RA receptors bound to RA response elements (RAREs) of which there are thousands in vertebrate genomes. Here, we combined chromatin immunoprecipitation sequencing (ChIP-seq) for epigenetic marks and RNA-seq on trunk tissue from wild-type and Aldh1a2-/- embryos lacking RA synthesis that exhibit body axis and forelimb defects. We identified a relatively small number of genes with altered expression when RA is missing that also have nearby RA-regulated deposition of histone H3

SUBMITTER: Berenguer M 

PROVIDER: S-EPMC7259794 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature