A new protocol for single-cell RNA-seq reveals stochastic gene expression during lag phase in budding yeast.

ABSTRACT: Current methods for single-cell RNA sequencing (scRNA-seq) of yeast cells do not match the throughput and relative simplicity of the state-of-the-art techniques that are available for mammalian cells. In this study, we report how 10x Genomics' droplet-based single-cell RNA sequencing technology can be modified to allow analysis of yeast cells. The protocol, which is based on in-droplet spheroplasting of the cells, yields an order-of-magnitude higher throughput in comparison to existing methods. After extensive validation of the method, we demonstrate its use by studying the dynamics of the response of isogenic yeast populations to a shift in carbon source, revealing the heterogeneity and underlying molecular processes during this shift. The method we describe opens new avenues for studies

SUBMITTER: Jariani A 

PROVIDER: S-EPMC7259953 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature