Pin1 promotes pancreatic cancer progression and metastasis by activation of NF-κB-IL-18 feedback loop.

ABSTRACT: OBJECTIVES:Accumulated evidence suggests that Pin1 contributes to oncogenesis of diverse cancers. However, the underlying mechanism of oncogenic function of Pin1 in PDAC requires further exploration. MATERIALS AND METHODS:IHC was performed using PDAC tissues. Western blot, PCR, immunofluorescence and transwell were performed using cell lines. GSEA were applied for possible downstream pathways. ChIP assay and dual luciferase were used for assessment of transcriptional activity. RESULTS:Both Pin1 and IL-18 levels are increased in primary PDAC tissues and that their levels are positively correlated. High expression of IL-18 is a predictor of poor prognoses. Pin1 promoted pancreatic cancer cell proliferation and motility by increasing IL-18 expression, while Pin1 knockdown also inhibited the t

SUBMITTER: Sun Q 

PROVIDER: S-EPMC7260075 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature